Virtually Everything You Wanted to Know about Citrate Transport: Computer Modeling Studies of a Membrane Protein
December 1, Tue 2009
1:00 pm, MRB 100 Conference Room
Dr. Eric Walters
Professor, Department of Biochemistry & Molecular Biology, Rosalind Franklin University of Medicine and Science, IL
Citrate plays a key role in both carbohydrate and lipid metabolism, both in the cytosol and in the mitochondrion. The mitochondrial citrate transporter is a membrane protein that facilitates movement of citrate between these compartments of the cell. I have used homology modeling to construct a three-dimensional model of this transporter (Walters and Kaplan, Biophys. J., 2004). This model has been used to rationalize results of single point mutation studies, to design new mutants for structure-function studies, and to identify probable citrate binding sites. More recently, it has been used to carry out virtual high throughput screening that has led to identification of specific inhibitors of citrate transport.