Friday, November 20, 2015
10:00 am, MRB 100C
Dr. Douglas M. Fowler
Department of Genome Sciences, University of Washington
Highly parallel measurement of the impact of mutations in proteins
Deep mutational scanning is a method that marries selection for protein function amongst a large library of protein variants with high-throughput DNA sequencing to measure the activity of hundreds of thousands of variants simultaneously. The result is a sequence-function map that describes the impact of all possible single and many double mutants on protein function. We have shown that sequence-function maps have many uses. For example, we are analyzing them to learn about protein properties like structure, aggregation, stability and enzyme mechanism; to guide the interpretation of coding variants in genomes; and to better understand protein evolution.