Domain motions in a tetrameric enzyme: modeling and experimental studies
Sep 25, Tue 2007
2:00 pm, MRB Conference Room
Dr. Krzysztof Kuczera
Departments of Chemistry and Molecular Biosciences, University of Kansas
I present our biochemical and modeling studies of the role of domain motions in the function of S-adenosyl-L-homocysteine hyrolase. The studies involve site-directed mutagenesis of residues in hinge regions, effects of reversible and irreversible inhibitors, fluorescence anisotropy decay, normal mode analysis and molecular dynamics simulations. What emerges is a complex and physically interesting picture of domain motions that is much richer than presented by any single approach.